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RNA Extraction & RT-PCR Kits

The Covid-19 RT-PCR test is for the qualitative detection of nucleic acid from SARS-CoV-2 in upper & lower respiratory specimens. Santé have a selection of RNA extraction and RT-PCR kits to enable this careful process of extraction, purification & detection.

Extraction_1

Extraction Kits

Qualitative detection & purification
The Viral RNA Extraction Kit is designed for the extraction & purification of nucleic acid from swab & saliva samples. It is available with 32 or 96 reactions/kit and utilises magnetic bead technology to bind the nucleic acid, wash the bound nucleic acid & subsequently elute the purified nucleic acid. The Viral RNA Extraction Kit can be run manually, automated with our Magec Instruments, or on the Thermo ScientificTM Kingfisher Flex Purification System.
RNA Extraction

Fortitude 2.1

Qualitative detection & purification
Fortitude Kit 2.1 is is a multiplex real-time PCR assay targeting the conserved regions ORF1ab region 1 (FAM) and ORF1ab region 2 (HEX) coupled with an internal spike-in
plasmid control. Fortitude Kit 2.1 has received CE Mark and regulatory approval in Singapore, Philippines, Panama, Peru, Colombia, and Honduras. The Limit of detection (LoD) is 10 copies per reaction, and the kit has no cross-reactivity with: SARS-CoV-1 (SARS, identified in 2003), Influenza A H1N1, Influenza A H3N2, Influenza B, MERS-CoV or EMC/2012 (MERS, identified in 2012).
PCR Assay

RT-PCR Kit

Amplification & data analysis

The COVID-19 Test-Kit is used for the qualitative detection via multiplex Real-Time PCR of 2019-nCoV specific RNA in nasopharyngeal swab samples. A positive test result from the test may indicate the presence of 2019-nCoV specific RNA in the test sample. There is no cross-reactivity with SARS, Influenza A H1N1, Influenza A H3N2, Influenza B, or MERS. "In terms of clinical sensitivity, the COVID-19 Test-Kit has been able to detect 100 percent of the positive samples tested, with no false positive & negatives reported".

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